Mycology and PhytopathologyMycology and PhytopathologyМикология и фитопатология0026-36483034-5421The Russian Academy of Sciences69623110.31857/S0026364825060036БИОРАЗНООБРАЗИЕ, СИСТЕМАТИКА, ЭКОЛОГИЯResearch ArticleDetection of Malassezia Fungi in Microplankton and Fishes in the Severnaya Bay, Sevastopol (Russia)Выявление грибов рода Malassezia в микропланктоне и на рыбах в северной бухте Севастополя (Россия)KolbasovN. M.КолбасовН. М.kiku200@yandex.ruPodkidyshevaYu. K.ПодкидышеваЮ. К.ShevyrevA. V.ШевыревА. В.KuznetsovA. V.КузнецовА. В.kuznet61@gmail.comSevastopol State UniversityСевастопольский государственный университетPirogov Russian National Research Medical UniversityРоссийский национальный исследовательский медицинский университет им. Н. И. Пирогова“DNA-technology” CompanyКомпания “ДНК-Технология”A. O. Kovalevsky Institute of Biology of the Southern Seas of the Russian Academy of SciencesИнститут биологии южных морей им. А. О. Ковалевского РАН15112025596VOL 59, NO6 (2025)ТОМ 59, №6 (2025)47748113112025Copyright ©; 2025, Russian Academy of SciencesCopyright ©; 2025, Российская академия наук2025Russian Academy of SciencesРоссийская академия наукhttps://oncopathology.abvpress.ru/0026-3648/article/view/696231

In this study, the microplankton and mycobiota of fish in Severnaya Bay, Sevastopol, was assessed using real-time PCR (RT-PCR). Microplankton samples were collected using a Biber-2 sequential filtration setup, and microbiota samples were obtained from the surface of fishes (Trachurus mediterraneus and Spicara smaris). DNA was extracted by alkaline lysis during high-temperature incubation and analyzed for the presence of 13 species of fungal pathogens from the genera Candida, Malassezia, Saccharomyces, and Debaryomyces. A positive result was obtained for Malassezia spp. in the planktonic fraction (150–300 µm), and a signal was detected from the surface of Trachurus mediterraneus. Other fungal pathogens, including Candida albicans, Debaryomyces hansenii, and Meyerozyma guilliermondii, were not detected. The results emphasize the role of microplankton and fish as potential reservoirs of fungal pathogens in marine ecosystems.

В рамках данного исследования была оценена микобиота микропланктона и рыб в Северной бухте г. Севастополя с использованием метода ПЦР в реальном времени. Пробы микропланктона были собраны с помощью установки последовательной фильтрации Biber-2, образцы микробиоты получены с поверхности рыб (ставрида Trachurus mediterraneus и окунь Spicara smaris). ДНК выделяли методом щелочного лизиса в ходе высокотемпературного инкубирования и анализировали на наличие 13 видов возбудителей грибных инфекций родов Candida, Malassezia, Saccharomyces и Debaryomyces. Получен положительный результат для Malassezia spp. в планктонной фракции (150–300 мкм) и сигнал с поверхности ставриды. Остальные возбудители грибных инфекций, включая Candida albicans, Debaryomyces hansenii и Meyerozyma guilliermondii, не выявлены. Результаты подчеркивают роль микропланктона и рыб как потенциальных резервуаров грибных патогенов в морских экосистемах.

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